Description
FIELD’S STAIN-B, also known as Field’s stain solution B, is a component of the Field’s staining method used in microbiology and histology for staining bacteria, especially acid-fast bacteria like Mycobacterium tuberculosis. This staining method is crucial for diagnosing tuberculosis and other related diseases.
Field’s stain solution B typically contains methylene blue or brilliant green, which serves as a counterstain to visualize non-acid-fast bacteria or background material. After staining with Field’s stain solution A (containing basic fuchsin) and decolorization, the sample is then counterstained with Field’s stain solution B to differentiate between acid-fast and non-acid-fast bacteria.